Expression cloning and radiotracer uptakes in Xenopus laevis oocytes

Nature Protocols 3, 1975 (2008). doi:10.1038/nprot.2008.151

Author: Daniel Markovich

This protocol describes the method of expression cloning of heterologous proteins using Xenopus laevis oocytes and the functional characterization of membrane proteins using radiotracer assays. It can be used to isolate proteins for which sequence data is unavailable and to characterize the functions of

img src="http://c.fsdn.com/fm/screenshots/49953_thumb.png" align="right" alt="Screenshot" hspace="10" vspace="10" Diskless Remote Boot in Linux (DRBL) provides a diskless or systemless environment for client machines. It works on Debian, Mandriva, Red Hat, Fedora, and SuSE. Unlike LTSP, it uses distributed hardware resources and makes it possible for clients to fully access local hardware. It also includes Clonezilla, a partitioning and disk cloning utility similar to Symantec Ghost. hr / strongLicense:/strong GNU General Public License (GPL) hr / strongChanges:/strongbr / Support for Ubuntu 8.10 and Mandriva 2009.0. New upstream memtest86+ 2.10. Some bugs were fixed. pa href="http://feedads.googleadservices.com/~a/cZYUZWrkhOL9rnQYXnAhSJnLa4I/a"img src="http://feedads.googleadservices.com/~a/cZYUZWrkhOL9rnQYXnAhSJnLa4I/i" border="0" ismap="true"/img/a/pimg src="http://feedproxy.google.com/~r/freshmeat/feeds/fm-releases-global/~4/uclfJmnXwUY" height="1" width="1"/

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Cloning saves storage because only one image of an application is shared across multiple users, according to VMware.

HowtoForge: "This tutorial shows how you can back up and restore hard drives and partitions with CloneZilla Live. CloneZilla Live is a Linux Live-CD that you insert into your computer; it contains hard disk and partition imaging and cloning tools similar to Norton Ghost. The created images are compressed and can be transferred to a Samba-, SSH-, or NFS server or to a local hard drive or USB drive."